Leaf Structure and Stomata

Middle schoolBiologySchematic three-dimensional leaf anatomy with a coupled steady-state CO₂ uptake and water-loss model

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About this experiment

Explore a leaf section and manipulate its guard-cell opening. Compare how carbon dioxide access, light and humidity affect photosynthetic uptake and water loss while identifying the functions of the main tissues.

Learning goals

  • Identify cuticle, epidermis, mesophyll, vascular tissue and stomata.
  • Explain the carbon-gain and water-loss tradeoff of opening pores.
  • Distinguish manual pore control from a complete physiological regulation model.

How to use it

  1. 1Click leaf layers and read their functions.
  2. 2Open and close the stomata while holding other conditions constant.
  3. 3Reduce light to zero and inspect uptake and water loss separately.
  4. 4Raise humidity to saturation and compare the water-use ratio.

The model

Internal CO₂ is solved from matching diffusion with uptake.
Water loss uses conductance and the vapour-pressure difference.

Assumptions

  • The cutaway separates tissue layers for inspection; it is not a scale anatomical reconstruction.
  • Conductance is 0.003 plus 0.2 times squared pore opening in mol air per square metre per second. Residual conductance represents unresolved surface pathways.
  • A steady diffusion balance solves internal CO₂ together with a saturating light and internal-CO₂ uptake law. Photosynthetic respiration and the external boundary layer are omitted.
  • Water-vapour conductance is 1.6 times the CO₂ conductance and uses the vapour-pressure difference at a total pressure of 101.3 kPa.
  • Pore opening is manual. The model does not automatically close stomata in darkness or respond to hormones or leaf water status.

Try this in class

  1. Q1Why can manually open pores lose water even when photosynthetic uptake is zero?
  2. Q2Why is a water-use ratio undefined when water flux is zero?

Frequently asked questions

Why is there no Run button?

The displayed anatomy and gas fluxes are a steady-state experiment. Moving a control immediately prepares another state.

Does an open pore always mean a higher net carbon gain?

This model reports photosynthetic uptake without respiration. It does not predict whole-plant net gain or all stresses.